NBRC 114121；Fusarium asiaticum O'Donnell et al.1.菌株基本信息
菌株其他名称：
有性型/Teleomorph：
无性型/Anamorph：
其他保藏机构编号：
其他编号：FGD7/FGS8
植物标本编号/Herberium No.：
提供形式：Micro tube (frozen-thawed)
模式菌株：
生物安全等级：L2
限制条款：Living Modified Organism (LMO)

使用条件：
植物检疫编号/Plant Quarantine No.：
动物检疫编号/Animal Quarantine No.：
菌株分离基物：Wheat grain, Triticum aestivum
采集地点：Ibaraki，Japan
接收日期：2019/08/20
分离年份：
保藏年份：2019
菌株来源历史/保藏单位：Meiji Univ. (K. Maeda, FGD7/FGS8)
备注：Trichothecene mycotoxins, 3,15-diacetylnivalenol, 3-acetylnivalenol, and 3-acetyldeoxynibalenol producing strain (tri7 and tri8 genes disruption mutant, and Fusarium graminearum tri8 gene over-expressing strain); pCSN43, pAnTef-neo: hygromycin B resistance gene, hph, ampicillin resistance gene, bla and G418 resistance gene, neo derived from Escherichia coli, trpC promoter, trpC terminator, and tef promoter from Aspergillus nidulans, gpd terminator from Cryphonectria parasitica, tri8 gene from F. graminearum; integrated into the host fungus Fusarium asiaticum MAFF 111233; poor formation of conidia.2.菌株培养生长条件
复苏液/Rehydration Fluid：
培养基/Medium：856，296
培养温度/Cultivation Temp.：20℃-30℃
需氧类型/Oxygen Relationship：好氧/Aerobe
使用者相关信息：Trichothecene mycotoxins, 3,15-diacetylnivalenol, 3-acetylnivalenol, and 3-acetyldeoxynibalenol producing strain (tri7 and tri8 genes disruption mutant, and Fusarium graminearum tri8 gene over-expressing strain); pCSN43, pAnTef-neo: hygromycin B resistance gene, hph, ampicillin resistance gene, bla and G418 resistance gene, neo derived from Escherichia℃oli, trpC promoter, trpC terminator, and tef promoter from Aspergillus nidulans, gpd terminator from℃ryphonectria parasitica, tri8 gene from F. graminearum; integrated into the host fungus Fusarium asiaticum MAFF 111233; poor formation of Conidia.3.序列信息
序列：tef1 LSU rDNA D1D2 ITS1-5.8S rDNA-ITS2
登录号/Accession No.；
生物样品ID号/Biosample ID：
生物项目ID号/Bioproject ID：4.其他信息
菌株主要用途：3,15-diacetylnivalenol production；3-acetylnivalenol production；3-acetyldeoxynivalenol production
交配型/Mating Type：
遗传标记/Genetic Markers：pCSN43, pAnTef-neo (hph, bla, neo, trpC promoter, trpC terminator, tef promoter, gpd terminator, tri8) [pCSN43, pAnTef-neo: hygromycin B resistance gene, hph, ampicillin resistance gene, bla and G418 resistance gene, neo from Escherichia coli, trpC promoter, trpC terminator, and tef promoter from Aspergillus nidulans, gpd terminator from Cryphonectria parasitica, tri8 gene from Fusarium graminearum]


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